Activation of three pathogen-inducible promoters of tobacco in transgenic pear (Pyrus communis L.) after abiotic and biotic elicitation.
Identifieur interne : 002520 ( Main/Exploration ); précédent : 002519; suivant : 002521Activation of three pathogen-inducible promoters of tobacco in transgenic pear (Pyrus communis L.) after abiotic and biotic elicitation.
Auteurs : Mickaël Malnoy [France] ; Jean-Stéphane Venisse ; Jean Paul Reynoird ; Elisabeth ChevreauSource :
- Planta [ 0032-0935 ] ; 2003.
Descripteurs français
- KwdFr :
- Activation de la transcription (effets des médicaments et des substances chimiques), Activation de la transcription (génétique), Bactéries (croissance et développement), Caulimovirus (croissance et développement), Contrainte mécanique (MeSH), Erwinia (croissance et développement), Esterases (génétique), Facteur de croissance végétal (pharmacologie), Glucuronidase (génétique), Glucuronidase (métabolisme), Immunité innée (génétique), Integrases (génétique), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Mutation (MeSH), Phytophthora (croissance et développement), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Protéines végétales (génétique), Pyrus (génétique), Pyrus (microbiologie), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes au cours du développement (effets des médicaments et des substances chimiques), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Tabac (génétique), Tabac (microbiologie), Transduction du signal (génétique), Transduction du signal (physiologie), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Bactéries, Caulimovirus, Erwinia, Phytophthora.
- effets des médicaments et des substances chimiques : Activation de la transcription, Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes végétaux.
- génétique : Activation de la transcription, Esterases, Glucuronidase, Immunité innée, Integrases, Maladies des plantes, Protéines de fusion recombinantes, Protéines végétales, Pyrus, Régions promotrices (génétique), Tabac, Transduction du signal.
- microbiologie : Maladies des plantes, Pyrus, Tabac.
- métabolisme : Glucuronidase, Protéines de fusion recombinantes.
- pharmacologie : Facteur de croissance végétal.
- physiologie : Transduction du signal.
- Contrainte mécanique, Mutation, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Bacteria (growth & development), Caulimovirus (growth & development), Erwinia (growth & development), Esterases (genetics), Gene Expression Regulation, Developmental (drug effects), Gene Expression Regulation, Plant (drug effects), Glucuronidase (genetics), Glucuronidase (metabolism), Immunity, Innate (genetics), Integrases (genetics), Mutation (MeSH), Phytophthora (growth & development), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Growth Regulators (pharmacology), Plant Proteins (genetics), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (genetics), Pyrus (genetics), Pyrus (microbiology), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), Signal Transduction (genetics), Signal Transduction (physiology), Stress, Mechanical (MeSH), Tobacco (genetics), Tobacco (microbiology), Transcriptional Activation (drug effects), Transcriptional Activation (genetics).
- MESH :
- chemical , genetics : Esterases, Glucuronidase, Integrases, Plant Proteins, Recombinant Fusion Proteins.
- drug effects : Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Transcriptional Activation.
- genetics : Immunity, Innate, Plant Diseases, Promoter Regions, Genetic, Pyrus, Signal Transduction, Tobacco, Transcriptional Activation.
- growth & development : Bacteria, Caulimovirus, Erwinia, Phytophthora.
- chemical , metabolism : Glucuronidase, Recombinant Fusion Proteins.
- microbiology : Plant Diseases, Pyrus, Tobacco.
- chemical , pharmacology : Plant Growth Regulators.
- physiology : Signal Transduction.
- Mutation, Plants, Genetically Modified, Stress, Mechanical.
Abstract
In order to improve pear resistance against fire blight caused by Erwinia amylovora, a search for promoters driving high-level expression of transgenes specifically in response to this bacterial pathogen has been undertaken. We have examined the ability of hsr203J, str246C and sgd24 tobacco (Nicotiana tabacum L.) promoters to drive expression of the uidA reporter gene in pear. Transgenic pear clones were obtained by Agrobacterium tumefaciens-mediated transformation. Beta-glucuronidase activity was determined quantitatively and qualitatively in these plants grown in vitro using fluorometric and histochemical assays and compared to cauliflower mosaic virus (CaMV) 35S promoter-driven activity. The hsr203J promoter appeared to be very weakly activated following inoculation in pear, which is the converse of the situation in tobacco. The str246C promoter was rapidly activated in pear during compatible and incompatible interactions, by wounding and following the application of several elicitors (capsicein, cryptogein, harpin, salicylic acid and jasmonic acid). The sgd24 promoter, a deletion derivative of str246C, exhibited a low level of expression after bacterial inoculation, was weakly activated by wounding and elicitors, and was not activated by phytohormones (salicylic acid and jasmonic acid). Interestingly, the sgd24 promoter was locally activated in pear, whereas the str246C promoter was activated systemically from the infection site. Taken together, these data show that, although the s tr246C and sgd24 promoters are less active than the CaMV35S promoter in pear, their pathogen-responsiveness would permit them to be used to drive the expression of transgenes to promote bacterial disease resistance.
DOI: 10.1007/s00425-002-0932-0
PubMed: 12624768
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Venisse">Jean-Stéphane Venisse</name></author><author><name sortKey="Reynoird, Jean Paul" sort="Reynoird, Jean Paul" uniqKey="Reynoird J" first="Jean Paul" last="Reynoird">Jean Paul Reynoird</name></author><author><name sortKey="Chevreau, Elisabeth" sort="Chevreau, Elisabeth" uniqKey="Chevreau E" first="Elisabeth" last="Chevreau">Elisabeth Chevreau</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12624768</idno><idno type="pmid">12624768</idno><idno type="doi">10.1007/s00425-002-0932-0</idno><idno type="wicri:Area/Main/Corpus">002558</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002558</idno><idno type="wicri:Area/Main/Curation">002558</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002558</idno><idno type="wicri:Area/Main/Exploration">002558</idno></publicationStmt><sourceDesc><biblStruct><analytic><title 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sortKey="Reynoird, Jean Paul" sort="Reynoird, Jean Paul" uniqKey="Reynoird J" first="Jean Paul" last="Reynoird">Jean Paul Reynoird</name></author><author><name sortKey="Chevreau, Elisabeth" sort="Chevreau, Elisabeth" uniqKey="Chevreau E" first="Elisabeth" last="Chevreau">Elisabeth Chevreau</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (growth & development)</term><term>Caulimovirus (growth & development)</term><term>Erwinia (growth & development)</term><term>Esterases (genetics)</term><term>Gene Expression Regulation, Developmental (drug effects)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Immunity, Innate (genetics)</term><term>Integrases (genetics)</term><term>Mutation (MeSH)</term><term>Phytophthora (growth & development)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Growth Regulators (pharmacology)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (genetics)</term><term>Pyrus (genetics)</term><term>Pyrus (microbiology)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>Signal Transduction (genetics)</term><term>Signal Transduction (physiology)</term><term>Stress, Mechanical (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (microbiology)</term><term>Transcriptional Activation (drug effects)</term><term>Transcriptional Activation (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription (effets des médicaments et des substances chimiques)</term><term>Activation de la transcription (génétique)</term><term>Bactéries (croissance et développement)</term><term>Caulimovirus (croissance et développement)</term><term>Contrainte mécanique (MeSH)</term><term>Erwinia (croissance et développement)</term><term>Esterases (génétique)</term><term>Facteur de croissance végétal (pharmacologie)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Immunité innée (génétique)</term><term>Integrases (génétique)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Mutation (MeSH)</term><term>Phytophthora (croissance et développement)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Pyrus (génétique)</term><term>Pyrus (microbiologie)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes au cours du développement (effets des médicaments et des substances chimiques)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Tabac (génétique)</term><term>Tabac (microbiologie)</term><term>Transduction du signal (génétique)</term><term>Transduction du signal (physiologie)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Esterases</term><term>Glucuronidase</term><term>Integrases</term><term>Plant Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Bactéries</term><term>Caulimovirus</term><term>Erwinia</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Developmental</term><term>Gene Expression Regulation, Plant</term><term>Transcriptional Activation</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Activation de la transcription</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Immunity, Innate</term><term>Plant Diseases</term><term>Promoter Regions, Genetic</term><term>Pyrus</term><term>Signal Transduction</term><term>Tobacco</term><term>Transcriptional Activation</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Bacteria</term><term>Caulimovirus</term><term>Erwinia</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Activation de la transcription</term><term>Esterases</term><term>Glucuronidase</term><term>Immunité innée</term><term>Integrases</term><term>Maladies des plantes</term><term>Protéines de fusion recombinantes</term><term>Protéines végétales</term><term>Pyrus</term><term>Régions promotrices (génétique)</term><term>Tabac</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Pyrus</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Pyrus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Facteur de croissance végétal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Plant Growth Regulators</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Transduction du signal</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Mutation</term><term>Plants, Genetically Modified</term><term>Stress, Mechanical</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Contrainte mécanique</term><term>Mutation</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to improve pear resistance against fire blight caused by Erwinia amylovora, a search for promoters driving high-level expression of transgenes specifically in response to this bacterial pathogen has been undertaken. We have examined the ability of hsr203J, str246C and sgd24 tobacco (Nicotiana tabacum L.) promoters to drive expression of the uidA reporter gene in pear. Transgenic pear clones were obtained by Agrobacterium tumefaciens-mediated transformation. Beta-glucuronidase activity was determined quantitatively and qualitatively in these plants grown in vitro using fluorometric and histochemical assays and compared to cauliflower mosaic virus (CaMV) 35S promoter-driven activity. The hsr203J promoter appeared to be very weakly activated following inoculation in pear, which is the converse of the situation in tobacco. The str246C promoter was rapidly activated in pear during compatible and incompatible interactions, by wounding and following the application of several elicitors (capsicein, cryptogein, harpin, salicylic acid and jasmonic acid). The sgd24 promoter, a deletion derivative of str246C, exhibited a low level of expression after bacterial inoculation, was weakly activated by wounding and elicitors, and was not activated by phytohormones (salicylic acid and jasmonic acid). Interestingly, the sgd24 promoter was locally activated in pear, whereas the str246C promoter was activated systemically from the infection site. Taken together, these data show that, although the s tr246C and sgd24 promoters are less active than the CaMV35S promoter in pear, their pathogen-responsiveness would permit them to be used to drive the expression of transgenes to promote bacterial disease resistance.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12624768</PMID><DateCompleted><Year>2003</Year><Month>06</Month><Day>20</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>216</Volume><Issue>5</Issue><PubDate><Year>2003</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Activation of three pathogen-inducible promoters of tobacco in transgenic pear (Pyrus communis L.) after abiotic and biotic elicitation.</ArticleTitle><Pagination><MedlinePgn>802-14</MedlinePgn></Pagination><Abstract><AbstractText>In order to improve pear resistance against fire blight caused by Erwinia amylovora, a search for promoters driving high-level expression of transgenes specifically in response to this bacterial pathogen has been undertaken. We have examined the ability of hsr203J, str246C and sgd24 tobacco (Nicotiana tabacum L.) promoters to drive expression of the uidA reporter gene in pear. Transgenic pear clones were obtained by Agrobacterium tumefaciens-mediated transformation. Beta-glucuronidase activity was determined quantitatively and qualitatively in these plants grown in vitro using fluorometric and histochemical assays and compared to cauliflower mosaic virus (CaMV) 35S promoter-driven activity. The hsr203J promoter appeared to be very weakly activated following inoculation in pear, which is the converse of the situation in tobacco. The str246C promoter was rapidly activated in pear during compatible and incompatible interactions, by wounding and following the application of several elicitors (capsicein, cryptogein, harpin, salicylic acid and jasmonic acid). The sgd24 promoter, a deletion derivative of str246C, exhibited a low level of expression after bacterial inoculation, was weakly activated by wounding and elicitors, and was not activated by phytohormones (salicylic acid and jasmonic acid). Interestingly, the sgd24 promoter was locally activated in pear, whereas the str246C promoter was activated systemically from the infection site. Taken together, these data show that, although the s tr246C and sgd24 promoters are less active than the CaMV35S promoter in pear, their pathogen-responsiveness would permit them to be used to drive the expression of transgenes to promote bacterial disease resistance.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Malnoy</LastName><ForeName>Mickaël</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Unité d'Amélioration des Espèces Fruitières et Ornementales, INRA, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Venisse</LastName><ForeName>Jean-Stéphane</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Reynoird</LastName><ForeName>Jean Paul</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Chevreau</LastName><ForeName>Elisabeth</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2002</Year><Month>11</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth Regulators</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C090432">STR246N protein, Nicotiana tabacum</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.-</RegistryNumber><NameOfSubstance UI="D019426">Integrases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="D004950">Esterases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="C088007">hsr203J protein, Nicotiana tabacum</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017796" MajorTopicYN="N">Caulimovirus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004885" MajorTopicYN="N">Erwinia</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004950" MajorTopicYN="N">Esterases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019426" MajorTopicYN="N">Integrases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031989" MajorTopicYN="N">Pyrus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013314" MajorTopicYN="N">Stress, Mechanical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2002</Year><Month>05</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2002</Year><Month>08</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>3</Month><Day>8</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>6</Month><Day>21</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>3</Month><Day>8</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12624768</ArticleId><ArticleId IdType="doi">10.1007/s00425-002-0932-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Pays de la Loire</li></region><settlement><li>Beaucouzé</li></settlement></list><tree><noCountry><name sortKey="Chevreau, Elisabeth" sort="Chevreau, Elisabeth" uniqKey="Chevreau E" first="Elisabeth" last="Chevreau">Elisabeth Chevreau</name><name sortKey="Reynoird, Jean Paul" sort="Reynoird, Jean Paul" uniqKey="Reynoird J" first="Jean Paul" last="Reynoird">Jean Paul Reynoird</name><name sortKey="Venisse, Jean Stephane" sort="Venisse, Jean Stephane" uniqKey="Venisse J" first="Jean-Stéphane" last="Venisse">Jean-Stéphane Venisse</name></noCountry><country name="France"><region name="Pays de la Loire"><name sortKey="Malnoy, Mickael" sort="Malnoy, Mickael" uniqKey="Malnoy M" first="Mickaël" last="Malnoy">Mickaël Malnoy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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